Journal: Molecular Medicine Reports

Article Title: NGR1 reduces neuronal apoptosis through regulation of ITGA11 following subarachnoid hemorrhage

doi: 10.3892/mmr.2025.13432

Figure Lengend Snippet: Western blot analysis validation of the bioinformatics results. (A) Protein expression levels of ITGA11, PI3K, Akt, p-Akt, BCL2 and BAX. (B) Densitometric analysis of the protein expression levels. Data are presented as the mean ± SD and were analyzed using a one-way ANOVA followed by a Tukey's honestly significant difference post hoc test. *P<0.05, **P<0.01, ***P<0.001. ns, not significant; P-, phosphorylated; SAH, subarachnoid hemorrhage; ITGA11, integrin subunit α11.

Article Snippet: Subsequently, the membranes were blocked in 5% skim milk powder (cat. no. SL1330; Beijing Coolaibo Technology Co., Ltd.) at room temperature for 2 h. The membranes were then incubated overnight at 4°C with the following primary antibodies: Cleaved-caspase 3 (1:2,000; cat. no. 9664S; Cell Signaling Technology, Inc.), caspase 3 (1:1,000; cat. no. 9662S; Cell Signaling Technology, Inc.), BAX (1:3,000; cat. no. 50599-2-Ig; ProteinTech Group, Inc.), BCL2 (1:3,000; cat. no. 68103-1-Ig; ProteinTech Group, Inc.), ITGA11 (1:1,000; cat. no. ab198826; Abcam), PI3K (1:1,000; cat. no. 4255S; Cell Signaling Technology, Inc.), Akt (1:1,000; cat. no. 9272S; Cell Signaling Technology, Inc.), phosphorylated (p)-Akt (1:1,000; cat. no. 4051S; Cell Signaling Technology, Inc.) and β-actin (1:20,000; cat. no. 20536-1-AP; ProteinTech Group, Inc.).

Techniques: Western Blot, Biomarker Discovery, Expressing

Journal: iScience

Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB

doi: 10.1016/j.isci.2024.108959

Figure Lengend Snippet:

Article Snippet: mouse anti-phosphorylated Akt monoclonal antibody , Santa Cruz Biotechnology , Cat# sc-514032; RRID: AB_2861344.

Techniques: Recombinant, Modification, Protease Inhibitor, Expressing, Clone Assay, Control, Generated, Knock-Out, Software